0172281 - UEB-Q 20023066 RIV NL eng J - Článek v odborném periodiku
Vláčilová, K. - Ohri, D. - Vrána, Jan - Čihalíková, Jarmila - Doleželová, Marie - Kahl, G. - Doležel, Jaroslav
Development of flow cytogenetics and physical genome mapping in chickpea (Cicer arietinum L.).
Chromosome Research. Roč. 10, č. 8 (2002), s. 695-706. ISSN 0967-3849. E-ISSN 1573-6849
Grant CEP: GA AV ČR IAA6038204; GA AV ČR IBS5038104
Výzkumný záměr: CEZ:AV0Z5038910
Klíčová slova: cell cycle * chickpea (Cicer arietinum) * human cytogenetics
Kód oboru RIV: EB - Genetika a molekulární biologie
Impakt faktor: 1.828, rok: 2002

Procedures for flow cytometric analysis and sorting of mitotic chromosomes (flow cytogenetics) have been developed for chickpea (Cicer arietinum). Suspensions of intact chromosomes were prepared from root tips treated to achieve a high degree of metaphase synchrony. The optimal protocol consisted of a treatment of roots with 2 mmol/L hydroxyurea for 18 h, a 4.5-h recovery in hydroxyurea-free medium, 2 h incubation with 10 micro mol/L oryzalin, and ice-water treatment overnight. This procedure resulted in an average metaphase index of 47
Trvalý link: http://hdl.handle.net/11104/0069320