0172272 - UEB-Q 20023057 RIV US eng J - Článek v odborném periodiku
Scherer, G. F. E. - Paul, R. U. - Holk, A. - Martinec, Jan
Down-regulation by elicitors of phosphatidylcholine-hydrolyzing phospholipase C and up-regulation of phospholipase A in plant cells.
Biochemical and Biophysical Research Communications. Roč. 293, č. 2 (2002), s. 766-770. ISSN 0006-291X. E-ISSN 1090-2104
Grant CEP: GA ČR GA522/00/1332
Výzkumný záměr: CEZ:AV0Z5038910
Klíčová slova: CULTURED PARSLEY CELLS * PHYTOALEXIN SYNTHESIS * OXIDATIVE BURST
Kód oboru RIV: CE - Biochemie
Impakt faktor: 2.935, rok: 2002

Phosphatidylcholine, labeled by two fluorescent fatty acids, was fed to cultured plant cells (Petrosilenum crispum, L.; VBI-0, Nicotiana benthiana, L.) and fluorescent diacylglycerol (DAG) was the major metabolite. When a glycoprotein elicitor, derived from Phytophthora sojae, was applied to the parsley cells and the small protein cryptogein from Phytophthora cryptogea was applied to the tobacco cells. these signal substances strongly and rapidly decreased the pool of fluorescent diacylglycerol and weakly increased the pool of free fluorescent fatty acid and of fluorescent lysophosphatidylcholine. The cells responded in a very similar way to the application of mastoparan, a wasp venom peptide. As phosphatidic acid was only a very minor fluorescent metabolite DAG is hypothesized to arise by the action of a phosphatidylcholine-hydrolyzing phospholipase C which was down-regulated by elicitors. Up-regulation of a phospholipase A by elicitors is also suggested by these results. This is the first evidence for phosphatidylcholine-hydrolyzing phospholipase C in plant signal transduction. (C) 2002 Elsevier Science (USA). All rights reserved.
Trvalý link: http://hdl.handle.net/11104/0069311