0172246 - UEB-Q 20023026 RIV CZ eng J - Článek v odborném periodiku
Šindelářová, Milada - Šindelář, Luděk - Burketová, Lenka
Glucose-6-phosphate dehydrogenase, ribonucleases and esterases upon tobacco mosaic virus infection and benzothiodiazole treatment in tobacco.
Biologia Plantarum. Roč. 45, č. 3 (2002), s. 423-432. ISSN 0006-3134. E-ISSN 1573-8264
Grant CEP: GA ČR GA522/99/1264
Výzkumný záměr: CEZ:AV0Z5038910
Klíčová slova: chlorophyll * Nicotiana tabacum * PAGE
Kód oboru RIV: EB - Genetika a molekulární biologie
Impakt faktor: 0.583, rok: 2002

Effect of the benzothiodiazole (BTH) pre-treatment was monitored during the acute infection stage in the susceptible and the hypersensitive tobacco plants infected with the tobacco mosaic virus (TMV). Dynamic changes in the contents of chlorophyll, the total proteins, and the pathogenesis related proteins (PR-proteins), and activities of ribonucleases (RNase), phosphomonoesterase (PME), phosphodiesterase (PDE), and glucose-6-phosphate dehydrogenase (G6P DH) were studied. Neither the protein nor the chlorophyll contents were significantly changed by the TMV infection and/or the BTH treatment. The BTH pre-treatment caused a substantial reduction in the multiplication of TMV in the locally-infected leaves of the hypersensitive cultivar Xanthi-nc (to 15.1 %). A lesser decrease (to 50.3 %) was observed in the locally-infected leaves of susceptible cultivar Samsun. But in the systemically-infected leaves of this cultivar, only a 4-d delay in the multiplication of TMV was found. In the locally-infected leaves of both cultivars, the activities of the RNase, PME, PDE and G6P DH were sharply increased during the acute phase of TMV multiplication (when compared with the healthy plants) and the curves of these activities correlated with the multiplication curves of TMV.
Trvalý link: http://hdl.handle.net/11104/0069285