0172184 - UEB-Q 20013063 RIV JP eng J - Článek v odborném periodiku
Žárský, Viktor - Soukupová, H.
Stress as a Trigger of Pollen Embryogenesis.
Korean Journal of Plant Tissue Culture. Roč. 27, č. 5 (2000), s. 411-413. ISSN 1015-5880
Grant CEP: GA AV ČR IAA5038907; GA ČR GA206/99/1138
Výzkumný záměr: CEZ:AV0Z5038910
Klíčová slova: Heat shock proteins * pollen embryogenesis * stress
Kód oboru RIV: EB - Genetika a molekulární biologie

The ability of microspores or young pollen grains (male gametophytes) to undergo developmetal switch to embryogenic (sporophytic) pathway exemplifies the concept of totipotency as applied to haploid postmeiotic cells. As a first step pollen is devoid of positional information provided in situ by the intact anther - by isolation and cultivation in vitro in artificial media. This is inevitably accompanied by some degree of stress response in microspore/pollen. lt has been shown in both monocots and dicots that intentional stress treatment (mostly starvation or heat shock) greatly stimulates embryo induction rate. Using transgenic sHSP antisense Nlicotiana tabacum we show that expression of smalt heat shock proteins is an integral part of successful embryo and later haploid plant production from pollen grains. Our recently published data show that sHSP chaperone function is optimal in the absence of ATP.
Trvalý link: http://hdl.handle.net/11104/0069226