Počet záznamů: 1  

Magnetic alginate microparticles for purification of .alpha.-amylases

  1. 1.
    0170718 - UEK-B 20033023 RIV NL eng J - Článek v odborném periodiku
    Šafaříková, Miroslava - Roy, I. - Gupta, M. N. - Šafařík, Ivo
    Magnetic alginate microparticles for purification of .alpha.-amylases.
    Journal of Biotechnology. Roč. 105, - (2003), s. 255-260. ISSN 0168-1656
    Grant CEP: GA MŠk OC 523.80; GA AV ČR IBS6087204
    Výzkumný záměr: CEZ:AV0Z6087904
    Klíčová slova: alginate * ferrofluid * amalyses
    Kód oboru RIV: CE - Biochemie
    Impakt faktor: 2.543, rok: 2003

    Spherical magnetic alginate microparticles (25 - 60 .micro.m in diameter) were prepared using the microemulsion system, with water saturated 1-pentanol as the organic phase. The limited solubility of 1-pentanol in water enabled simple removal of the organic solvent from the prepared beads with water solutions. The prepared alginate microparticles were used as magnetic affinity adsorbents for specific purification of .alpha.-amylases. Enzyme activity was eluted by 1.0 M maltose. Alpha-amylases from Bacillus amyloliquefaciens and porcine pancreatic acetone powder were purified 9- and 12-fold with 88 and 96 % activity recovery, respectively.
    Trvalý link: http://hdl.handle.net/11104/0067800