0162255 - UIACH-O 20010009 RIV NL eng J - Článek v odborném periodiku
Horká, Marie - Willimann, T. - Blum, M. - Nording, P. - Friedl, Z. - Šlais, Karel
Capillary isoelectric focusing with UV-induced fluorescence detection.
Journal of Chromatography A. Roč. 916, 1-2 (2001), s. 65-71. ISSN 0021-9673. E-ISSN 1873-3778
Grant CEP: GA AV ČR IAA4031901
Výzkumný záměr: CEZ:AV0Z4031919
Klíčová slova: isoelectric focusing * detection * electrophoresis
Kód oboru RIV: CB - Analytická chemie, separace
Impakt faktor: 2.793, rok: 2001

Low-molecular-mass fluorescent compounds excitable in the near UV region with suitable acidobasic and electrophoretic properties are suggested as isoelectric point (pI) markers for isoelectric focusing (IEF) with UV excited fluorometric detection and properties of new UV-induced fluorescent pI markers are given. The PI values of 18 new pI markers determined independently of IEF methods range from 2.1 to 10.3 The examples of separation of new pI markers together with derivatized proteins by capillary IEF with photometric or fluorometric detection are presented.
Trvalý link: http://hdl.handle.net/11104/0059574