0162175 - UIACH-O 200005 RIV DE eng J - Článek v odborném periodiku
Klepárník, Karel - Malá, Zdeňka - Přibyla, Lubomír - Blažková, M. - Vašků, A. - Boček, Petr
Ultrafast detection of microsatellite repeat polymorphism in endothelin 1 gene by electrophoresis in short capillaries.
Electrophoresis. Roč. 21, č. 1 (2000), s. 238-246. ISSN 0173-0835. E-ISSN 1522-2683
Grant CEP: GA ČR GA203/97/0347; GA ČR GA301/97/1192; GA AV ČR IAA4031703; GA MŠMT VS96021
Kód oboru RIV: CB - Analytická chemie, separace
Impakt faktor: 3.385, rok: 2000

The methodology and instrumentation for fast denaturing electrophoresis in short capillaries has been developed and exemplified by detection of short tandem repeat polymorphism in endothelin 1 gene. The resolution of two nucleotides, which is required for the detection of a dinucleotide repeat polymorphism, was achieved in a capillary of an effective length of 2.5 cm at a temperature of 60oC and an electric field strength of 600 V/cm in 42 seconds.
Trvalý link: http://hdl.handle.net/11104/0059495