0142471 - FGU-C 20030080 RIV US eng J - Článek v odborném periodiku
He, M. L. - Zemková, Hana - Koshimizu, T. - Tomič, M. - Stojilkovic, S. S.
Intracellular calcium measurements as a method in studies on activity of purinergic P2X receptor channels.
American Journal of Physiology-Cell Physiology. Roč. 285, č. 2 (2003), s. C467-C479. ISSN 0363-6143. E-ISSN 1522-1563
Výzkumný záměr: CEZ:AV0Z5011922
Klíčová slova: P2X purinergic receptor * calcium signaling * membrane current
Kód oboru RIV: ED - Fyziologie
Impakt faktor: 4.103, rok: 2003

Spatiotemporal characteristics of intracellular Ca2+ signaling and its dependence on current signaling was studied in excitable mouse immortalized gonadotropin-releasing hormone-secreting cells (GT1) and nonexcitable human embryonic kidney cells (HEK-293) cells expressing wild-type and chimeric P2XRs. The temporal characteristics of Ca2+ signals reflected well the receptor-specific EC50 values for ATP and the extracellular Zn2+ and pH sensitivities of P2XRs. These results indicate that intracellular Ca2+ measurements are useful for characterizing the pharmacological and kinetic properties of P2XRs, when the host cells do not express other members of purinergic receptors.
Trvalý link: http://hdl.handle.net/11104/0040174