Cytological changes and alterations in polyamine contents induced by cadmium in tobacco BY-2 cells

0109155 - UEB-Q 20043078 RIV FR eng J - Článek v odborném periodiku
Kuthanová, A. - Gemperlová, Lenka - Zelenková, S. - Eder, Josef - Macháčková, Ivana - Opatrný, Z. - Cvikrová, Milena
Cytological changes and alterations in polyamine contents induced by cadmium in tobacco BY-2 cells.
[Cytologické změny a změny v obsazích polyaminů v buňkách tabáku BY-2 indukovaných kadmiem.]
Plant Physiology and Biochemistry. Roč. 42, č. 2 (2004), s. 149-156. ISSN 0981-9428
Grant CEP: GA MŠMT LN00A081
Výzkumný záměr: CEZ:AV0Z5038910
Klíčová slova: BY-2 cells * cadmium * DAO
Kód oboru RIV: EF - Botanika
Impakt faktor: 1.414, rok: 2004

Changes in cell viability, proliferation, cell and nuclear morphology including nuclear and DNA fragmentation induced by 0.05 and 1 mM CdSO4 (Cd2+) in tobacco BY-2 cell line (Nicotiana tabacum L.) were studied in the course of 7 days. Simultaneously changes in endogenous contents of both free and conjugated forms of polyamines (PAs) were investigated for 3 days. The application of 0.05 mM Cd2+ evoked decline of cell viability to approximately 60% during the first 24 h of treatment. Later on degradation of cytoplasmic strands, formation of the stress granules and vesicles. modifications in size and shape of the nuclei, including their fragmentation. were observed in the surviving cells. Their proliferation was blocked and cells elongated. Beginning the first day of treatment TUNEL-positive nuclei were detected in cells cultivated in medium containing 0.05 mM Cd2+. Treatment with highly toxic 1 mM Cd2+ induced fast decrease of cell viability (no viable cells remained after 6-h treatment) and cell death occurred before DNA cleavage might be initiated. The exposure of tobacco BY-2 cells to 0.05 mM Cd2+ resulted in a marked accumulation of total PAs (represented by the sum of free PAs and their perchloric acid (PCA)-soluble and PCA-insoluble conjugates) during 3-day treatment. The increase in total PA contents was primarily caused by the increase in putrescine (Put) concentration. The accumulation of free spermidine (Spd) and spermine (Spm) at 12 and 24 h in 0.05 mM Cd2+ treated BY-2 cells and high contents of Spd and especially Spm determined in dead cells after 1 mM Cd2+ application was observed. The participation of PA conjugation with hydroxycinnamic acids and PA oxidative deamination in maintaining of free PA levels in BY-2 cells under Cd2+-induced oxidative stress is discussed.

V buněčné suspenzní kultuře tabáku BY-2 byly studovány změny v buněčné viabilitě, proliferaci, buněčné a jaderné morfologii včetně jadernéa DNK fragmentace indukovaných působením kadmia po dobu 7 dnů. Současně byly sledovány změny v obsazích volných a konjugovaných forem polyaminů po dobu 3 dnů. Aplikace kadmia snížila buněčnou viabilitu jiz po 24 hodinách na 60% ve srovnání s kontrolou. Při delším působení dochází k rozpadu cytoplasmatických provazců, k tvorbě stresových granulí a mění se velikost a tvar jádra. Vystavení buněk tabáku BY-2 účinkům kadmia po dobu 3 dnů vyvolalo výrazný nárůst hladiny polyaminů, především putrescinu.
Trvalý link: http://hdl.handle.net/11104/0016265