0105015 - UCHP-M 20040269 RIV NL eng J - Článek v odborném periodiku
Kuncová, Gabriela - Podrazký, Ondřej - Ripp, S. - Trögl, Josef - Sayler, G. S. - Demnerová, K. - Vaňková, Radomíra
Monitoring of the Viability of Cells Immobilized by Sol-Gel Process.
[Sledování viability buněk imobilizovaných procesem sol-gel.]
Journal of Sol-Gel Science and Technology. Roč. 31, 1-3 (2004), s. 335-342. ISSN 0928-0707. E-ISSN 1573-4846.
[International Workshop on Sol-Gel and Technology-Part I (Sol-Gel'03) /12./. Sydney, 25.08.2003-29.08.2003]
Grant CEP: GA ČR GA104/01/0461; GA MŠMT OC 840.20; GA MŠMT OC 840.10
Výzkumný záměr: CEZ:AV0Z4072921
Klíčová slova: sol-gel process * cell entrapment * viability
Kód oboru RIV: CE - Biochemie
Impakt faktor: 1.150, rok: 2004

Three different types of cells, Pseudomonas fluorescens HK44, Saccharomyces cerevisiae strain SP4 and plant cells Nicotiana tabacum L. BY-2, were immobilized by entrapment in tetramethoxysilane prepolymer (TMOS) gel or in composite gel containing prepolymer TMOS and alginate in various ratios. Their growth and viability were monitored by bioluminescence and 2-D fluorescence spectra, which are fast and do not need the dissolution of a matrix. The resulting biocomposite gels were obtained by gelation of the mixtures of TMOS prep. or TMOS/alginate sols and the particular cells in proper media on glass supports to provide films ~1 mm thick. The effect of the following parameters on the growth and viability of the cells was studied: (a) the composition of the biocomposites, (b) the preparation conditions of TMOS and (c) the conditions of the procedure of entrapment. All three types of cells were tested in TMOS gel and the composite TMOS/alginate = 1:1 (v/v).

Tři různé typy buněk, Pseudomonas fluorescens HK44, Saccharomyces cerevisiae strain SP4 and plant cells Nicotiana tabacum L. BY-2, byly imbilizovány zachycením v předpolymerizovaném tetramethoxysilanu a kompositním gelu složeném z předpolymerovaného teramethoxysilanu a alginatu. Viabilita bakterii byla ve srovnáním s kvasinkami a rostlinými buňkami snížna v důsledku imobilizace nejméně.
Trvalý link: http://hdl.handle.net/11104/0012268