Papaya ringspot virus coat protein gene for antigen presentation Escherichia coli

0098694 - ÚEB 2008 RIV KR eng J - Článek v odborném periodiku
Chatchen, S. - Juříček, Miloslav - Rueda, P. - Kertbundit, Sunee
Papaya ringspot virus coat protein gene for antigen presentation Escherichia coli.
[Papaya ringspot virus coat protein gene for antigen presentation Escherichia coli.]
Journal of Biochemistry and Molecular Biology. Roč. 39, č. 1 (2006), s. 16-21. ISSN 1225-8687
Grant ostatní: Thai Research Fund(TH) BT-B-06-PG-14-4503
Výzkumný záměr: CEZ:AV0Z50380511
Zdroj financování: V - jiné veřejné zdroje
Klíčová slova: antigen presentation * canine parvo virus * epitope * papaya ringspot virus
Kód oboru RIV: EF - Botanika
Impakt faktor: 1.465, rok: 2006
http://www.jbmb.or.kr/view_article.php3?cont=jbmb&kid=174&mid=3&pid=3

The coat protein (CP) of Papaya ringspot virus (PRSV) was analyzed for presentation of the antigenic peptide of animal virus, Canine parvovirus (CPV), in Escherichia coli (E. coli). The 45 nucleotides fragment coding for the 15-aa peptide epitope of the CPV-VP2 protein was either inserted into the PRSV-cp gene at the 5', 31 ends, both 5' and 3' ends or substituted into the 3' end of the PRSV cp gene. Each of the chimeric PRSV cp genes was cloned into the pRSET B vector under the control of the T7 promoter and transformed into E. coli. The recombinant coat proteins expressed from different chimeric PRSV-cp genes were purified and intraperitoneally injected into mice. All of the recombinant coat proteins showed strong immunogenicity and stimulate mice immune response. The recombinant coat proteins containing the CPV epitope insertion at the C terminus and at both N and C termini elicited ten times higher specific antisera in immunized mice compared with the other two recombinant coat proteins which contain the CPV epitope insertion at the N terminus and substitution at the C terminus.

Papaya ringspot virus coat protein gene for antigen presentation Escherichia coli.
Trvalý link: http://hdl.handle.net/11104/0157540