0028430 - ÚEB 2006 RIV GR eng J - Článek v odborném periodiku
Pokorná, D. - Čeřovská, Noemi - Šmahel, M. - Moravec, Tomáš - Ludvíková, V. - Machková, J. - Synková, Helena - Dušková, M. - Hozák, P. - Velemínský, Jiří
DNA vaccines based on chimeric potyvirus-like particles carrying HPV16 E7 peptide (aa 44-60).
[DNA vakcinace založená na chimérických viru podobných částicích polyvirů nesoucích HPV 16 E7 peptid (aa44-66).]
Oncology Reports. Roč. 14, č. 4 (2005), s. 1045-1053. ISSN 1021-335X. E-ISSN 1791-2431
Grant CEP: GA ČR GA310/00/0381
Grant ostatní: IGA MHCR NC7552-3/2003
Výzkumný záměr: CEZ:AV0Z50380511
Klíčová slova: human papillomavirus * E7 * virus-like particles
Kód oboru RIV: EI - Biotechnologie a bionika
Impakt faktor: 1.572, rok: 2005

Vaccine strategies for the treatment of humen papillomavirus-induced cervical cancer are based maily on the humen papillomavirus 16 E7 (HPV 16 E7) oncoprotein. The immunogenicity of the E7 gene has been enhanced by its fusion to many different genes. Here, we linked a short sequence coding for the E7 peptide (aa 44-60) containing immunodominant epitopes for B and T cells to the 3´ end of the gene coding for the whole coat protein (CP) of the potyvirus, potato virus A, and its deleted form (CPdel) with a short C-terminal deletion of 5 amino acids (LGVKG). CP-E7 and CPdel-E7 fusion proteins, just like CP alone, spotaneously assembled into virus-like particles in both procaryotic and eucaryotic cells. The CP-E7 and CPdel-7 induced slightly stronger E7-specific cytotoxin T-lymphocyte response than the whole E7 gene, although they were still lower than those elicited by the previously consctructed fusion gene, Sig/E7GGG/LAMP-1. The E-7 and CP-specific antibody responses were not detected in mice vaccinated with CP-E7 and CPdel-E7 fusion genes. The CP-E7 and CPdel-E7 fusion genes protected mise against the development of tumors induced by TC-1 cells producing the E7 antigen and were also effetive in the therapeutic setting, i.e. when the wacciation was performed after tumor cell administration. Their antitumor effect were comparable to those of the whole E7 gene and Sig/E7GGG/LAMP-1 fusion gene. There were no relevant difference between immune respopnses elicited by CP-E7 and CPdel-E7 DNA vaccination.

Geny pro obalový protein A viru bramboru (PVACP) a pro různé kombinace PVACP + epitopy ze strukturních a nestrukturních proteinů (E7 +L2) lidského papilloma viru HPV-16 byly vloženy do vektorového systému založeného X viru bramboru (PVX), kde tvořily viru podobné částice. Uvedené konstrukty byly použity k vakcinaci laboratorních zvířat, kdy protilátková odpověď nebyla detekována, a k DNA vakcinaci, kdy se projevila ochrana myší před tumory vyvolanými buněčnou nádorovou linií TC-1.
Trvalý link: http://hdl.handle.net/11104/0118371