0027287 - BÚ 2010 RIV CZ eng J - Článek v odborném periodiku
Záveský, L. - Jarolímová, Vlasta - Štěpánek, Jan
Nuclear DNA content variation within the genus Taraxacum (Asteraceae).
[Rozdíly v obsahu nukleární DNK uvnitř r. Taraxacum (Asteraceae).]
Folia Geobotanica. Roč. 40, - (2005), s. 91-104. ISSN 1211-9520. E-ISSN 1874-9348
Grant CEP: GA ČR(CZ) GD206/03/H137; GA ČR(CZ) GA206/05/0970
Výzkumný záměr: CEZ:AV0Z60050516
Klíčová slova: Taraxacum * C-value * genome size
Kód oboru RIV: EF - Botanika
Impakt faktor: 1.033, rok: 2005

Nuclear DNA content was estimated using flow cytometry in 13 sections represented by 18 species of the genus Taraxacum using propidium iodide as the DNA stain. Investigated plants represented diploid, triploid and tetraploid species from sections considered both primitive and advanced, i.e. Dioszegia, Piesis, Glacialia, Mongolica, Scariosa, Obovata, T. pyrenaicum group, T. sect. Coronata, Palustria, Taraxacum (= Crocea), Kashmirana, Ruderalia and Erythrosperma. Estimated nuclear 2C DNA content ranged from 1.74 pg in diploid T. linearisquameum (sect. Ruderalia) to 6.91 pg in tetraploid T. albidum (T. sect. Mongolica), demonstrating 3.97-fold variation. The lowest monoploid genome size 1Cx = 0.87 pg was recorded in T. linearisquameum (T. sect. Ruderalia) together with T. brachyglossum (T. sect. Erythrosperma), and the highest one (1.73 pg) was recorded in T. albidum (T. sect. Mongolica), giving 1.99-fold difference in the genus. No significant differences in genome size were observed within T. sect. Ruderalia, similarly no intraspecific variation was observed in T. paludosum (T. sect. Palustria) and T. serotinum (T. sect. Dioszegia). These results indicate a high intraspecific stability of the trait. Preliminary comparisons of genome size in species/sections considered to be close relatives were made. These data give a tentative additional evidence for the close phylogenetic relationship between sections Palustria and Piesis and against the close relationship between sections Piesis and Dioszegia.

Obsah nukleární DNK byl zjištěn pomocí průtokové cytometrie u 18 druhů z 13 sekcí rodu Taraxacum (T. sect. Dioszegia, Piesis, Glacialia, Mongolica, Scariosa, Obovata, T. pyrenaicum group, T. sect. Coronata, Palustria, Taraxacum (= Crocea), Kashmirana, Ruderalia and Erythrosperma). Obsah 2C DNA se pohybuje v rozmezí od 1.74 pg (T. linearisquameum) do 6.91 pg (T. albidum). Velikost genomu vykazuje velkou intraspecifickou stabilitu, nebyly pozorovány významné rozdíly uvnitř sect. Ruderalia, u T. paludosum (sect. Palustria) a T. serotinum (sect. Dioszegia). Předběžné porovnání velikosti genomu ukazuje na fylogenetickou příbuznost sect. Palustria se sect. Piesis a sect. Piesis se sect. Dioszegia.
Trvalý link: http://hdl.handle.net/11104/0003250