0025809 - ÚFCH JH 2006 RIV FR eng J - Článek v odborném periodiku
Gielen, E. - Vercammen, J. - Sýkora, Jan - Humpolíčková, Jana - van de Ven, M. - Benda, Aleš - Hellings, N. - Hof, Martin - Engelborghs, Y. - Steels, P. - Ameloot, M.
Diffusion of sphingomyelin and myelin oligodendrocyte glycoprotein in the membrane of OLN-93 oligodendroglial cells studied by fluorescence correlation spectroscopy.
[Difúze sfingomyelinu a myelinového proteinu oligodendrocytů v membráně OLN-93 oligodendrogliáních buněk studovaná pomocí fluorescenční korelační spektroskopie.]
Comptes Rendus Biologies. Roč. 328, č. 12 (2005), s. 1057-1064. ISSN 1631-0691. E-ISSN 1768-3238
Výzkumný záměr: CEZ:AV0Z40400503
Klíčová slova: OLN-93 * FCS * MOG * sphingomyelin
Kód oboru RIV: CF - Fyzikální chemie a teoretická chemie
Impakt faktor: 1.199, rok: 2005

Evidence has been accumulated that the plasma membrane of various mammalian cell types is heterogeneous in structure and may contain lipid microdomains (lipid rafts). This study focuses on the membrane organization of living oligodendrocytes, which are the myelin-producing cells of the central nervous system. Fluorescence correlation spectroscopy (FCS) was used to monitor the lateral diffusion of a lipid and of a protein in the oligodendroglial cell line OLN-93. The lipid was fluorescently labelled sphingomyelin (Bodipy FL-C5 SM). The protein was the myelin oligodendrocyte glycoprotein (MOG). In order to monitor the lateral diffusion of MOG, OLN-93 cells were transfected with a MOG-EGFP (enhanced green fluorescent protein) fusion plasmid. The measurements were performed at room temperature. FCS data were analyzed for two-dimensional (2D) diffusion according to three models which all included a triplet fraction: (a) 2D I component (2D1C), (b) ...

V této práci byla studována difúze sfingomyelinu a myelinového proteinu oligodendrocytů v membráně OLN-93 oligodendrogliáních buněk studovaná pomocí fluorescenční korelační spektroskopie.
Trvalý link: http://hdl.handle.net/11104/0116153