Počet záznamů: 1  

Concurrent Compression of Phospholipid Membranes by Calcium and Cholesterol

  1. 1.
    SYSNO ASEP0517192
    Druh ASEPJ - Článek v odborném periodiku
    Zařazení RIVJ - Článek v odborném periodiku
    Poddruh JČlánek ve WOS
    NázevConcurrent Compression of Phospholipid Membranes by Calcium and Cholesterol
    Tvůrce(i) Melcrová, Adéla (UFCH-W)
    Pokorná, Šárka (UFCH-W) RID
    Vošahlíková, Miroslava (FGU-C) RID, ORCID, SAI
    Sýkora, Jan (UFCH-W) RID
    Svoboda, Petr (FGU-C) RID, ORCID
    Hof, Martin (UFCH-W) RID, ORCID
    Cwiklik, Lukasz (UFCH-W) RID, ORCID
    Jurkiewicz, Piotr (UFCH-W) RID, ORCID
    Zdroj.dok.Langmuir. - : American Chemical Society - ISSN 0743-7463
    Roč. 35, č. 35 (2019), s. 11358-11368
    Poč.str.11 s.
    Jazyk dok.eng - angličtina
    Země vyd.US - Spojené státy americké
    Klíč. slovahek293 cells ; bilayer-membranes ; divalent-cations ; lipid-bilayers ; fusion protein ; binding ; hydration ; fluorescence ; dynamics ; sodium
    Vědní obor RIVCF - Fyzikální chemie a teoretická chemie
    Obor OECDPhysical chemistry
    Vědní obor RIV – spolupráceFyziologický ústav - Biochemie
    CEPGA17-05903S GA ČR - Grantová agentura ČR
    GA18-26751S GA ČR - Grantová agentura ČR
    Způsob publikováníOmezený přístup
    Institucionální podporaUFCH-W - RVO:61388955 ; FGU-C - RVO:67985823
    UT WOS000484644000015
    EID SCOPUS85071784813
    DOI10.1021/acs.langmuir.9b00477
    AnotaceRegulation of cell metabolism, membrane fusion, association of proteins with cellular membranes, and cellular signaling altogether would not be possible without Ca2+ ions. The distribution of calcium within the cell is uneven with the negatively charged inner leaflet of the plasma membrane being one of the primary targets of its accumulation. Therefore, we decided to map the influence of Ca2+ on the properties of lipid bilayers closely resembling natural lipid membranes. We combined fluorescence spectroscopy (analysis of time-resolved emission spectra of Laurdan probe and derived parameters: integrated relaxation time related to local lipid mobility, and total emission shift reflecting membrane polarity and hydration) with molecular dynamics simulations to determine the effect of the increasing CaCl2 concentration on model lipid membranes containing POPC, POPS, and cholesterol. On top of that, the impact of calcium on the plasma membranes isolated from HEK293 cells was investigated using the steady-state fluorescence of Laurdan. We found that calcium increases rigidity of all the model lipid membranes used, elevates their thickness, increases lipid packing and ordering, and impedes the local lipid mobility. All these effects were to a great extent similar to those elicited by cholesterol. However, the changes of the membrane properties induced by calcium and cholesterol seem largely independent from each other. At sufficiently high concentrations of calcium or cholesterol, the steric effects hindered a further alteration of membrane organization, i.e., the compressibility limit of membrane structures was reached. We found no indication for mutual interaction between Ca2+ and cholesterol, nor competition of Ca2+ ions and hydroxyl groups of cholesterol for binding to phospholipids. Fluorescence measurements indicated that Ca2+ adsorption decreases mobility within the carbonyl region of model bilayers more efficiently than monovalent ions do (Ca2+ >> Li+ > Na+ > K+ > Cs+). The effects of calcium ions were to a great extent mitigated in the plasma membranes isolated from HEK293 cells when compared to the model lipid membranes. Noticeably, the plasma membranes showed remarkably higher resistance toward rigidification induced by calcium ions even when compared with the model membranes containing cholesterol.
    PracovištěÚstav fyzikální chemie J.Heyrovského
    KontaktMichaela Knapová, michaela.knapova@jh-inst.cas.cz, Tel.: 266 053 196
    Rok sběru2020
    Elektronická adresahttp://hdl.handle.net/11104/0302482
Počet záznamů: 1  

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