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CKS1 Germ Line Exclusion Is Essential for the Transition from Meiosis to Early Embryonic Development
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SYSNO ASEP 0512107 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název CKS1 Germ Line Exclusion Is Essential for the Transition from Meiosis to Early Embryonic Development Tvůrce(i) Ellederová, Zdeňka (UZFG-Y) RID, ORCID
del Rincon, S. (US)
Končická, Markéta (UZFG-Y) ORCID
Šušor, Andrej (UZFG-Y) RID, ORCID
Kubelka, Michal (UZFG-Y) RID, ORCID
Sun, D. (US)
Spruck, C. (US)Číslo článku UNSP e00590-18 Zdroj.dok. Molecular and Cellular Biology. - : American Society for Microbiology - ISSN 0270-7306
Roč. 39, č. 13 (2019)Poč.str. 18 s. Forma vydání Online - E Jazyk dok. eng - angličtina Země vyd. US - Spojené státy americké Klíč. slova CKS ; cyclin dependent kinases ; developmental biology Vědní obor RIV EB - Genetika a molekulární biologie Obor OECD Cell biology CEP LO1609 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy EF15_003/0000460 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy GA15-22765S GA ČR - Grantová agentura ČR GA18-19395S GA ČR - Grantová agentura ČR Způsob publikování Open access Institucionální podpora UZFG-Y - RVO:67985904 UT WOS 000471243000005 EID SCOPUS 85068118528 DOI 10.1128/MCB.00590-18 Anotace Cell division cycle (cdc) kinase subunit (CKS) proteins bind cyclin-dependent kinases (CDKs) and play important roles in cell division control and development, though their precise molecular functions are not fully understood. Mammals express two closely related paralogs called CKS1 and CKS2, but only CKS2 is expressed in the germ line, indicating that it is solely responsible for regulating CDK functions in meiosis. Using cks2(-/-) knockout mice, we show that CKS2 is a crucial regulator of maturation-promoting factor (MPF, CDK1-cyclin A/B) activity in meiosis. cks2(-/-) oocytes display reduced and delayed MPF activity during meiotic progression, leading to defects in germinal vesicle breakdown (GVBD), anaphase-promoting complex/cyclosome (APC/C) activation, and meiotic spindle assembly. cks2(-/-) germ cells express significantly reduced levels of the MPF components CDK1 and cyclins A1/B1. Additionally, injection of MPF plus CKS2, but not MPF alone, restored normal GVBD in cks2(-/- )oocytes, demonstrating that GVBD is driven by a CKS2-dependent function of MPF. Moreover, we generated cks2(cks1/cks1)( )knock-in mice and found that CKS1 can compensate for CKS2 in meiosis in vivo, but homozygous embryos arrested development at the 2- to 5-cell stage. Collectively, our results show that CKS2 is a crucial regulator of MPF functions in meiosis and that its paralog, CKS1, must be excluded from the germ line for proper embryonic development. Pracoviště Ústav živočišné fyziologie a genetiky Kontakt Jana Zásmětová, knihovna@iapg.cas.cz, Tel.: 315 639 554 Rok sběru 2020 Elektronická adresa https://mcb.asm.org/content/39/13/e00590-18
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