Počet záznamů: 1

Silencing of carbonic anhydrase I enhances the malignant potential of exosomes secreted by prostatic tumour cells

SYSNO ASEP	0509053
Druh ASEP	J - Článek v odborném periodiku
Zařazení RIV	J - Článek v odborném periodiku
Poddruh J	Článek ve WOS
Název	Silencing of carbonic anhydrase I enhances the malignant potential of exosomes secreted by prostatic tumour cells
Tvůrce(i)	Bánová Vulić, R. (SK) Zdurienčíková, M. (SK) Tyčiaková, S. (SK) Benada, Oldřich (MBU-M)_{ORCID, RID} Dubrovčáková, M. (SK) Lakota, J. (SK) Škultéty, L'udovít (MBU-M)_{ORCID, RID}
Zdroj.dok.	Journal of Cellular and Molecular Medicine. - : Wiley - ISSN 1582-1838 Roč. 23, č. 5 (2019), s. 3641-3655
Poč.str.	15 s.
Jazyk dok.	eng - angličtina
Země vyd.	US - Spojené státy americké
Klíč. slova	carbonic anhydrase I ; LC-MS ; malignant potential
Vědní obor RIV	EE - Mikrobiologie, virologie
Obor OECD	Microbiology
CEP	LO1509 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy
	8X17052 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy
Způsob publikování	Open access
Institucionální podpora	MBU-M - RVO:61388971
UT WOS	000466841100055
EID SCOPUS	85064815688
DOI	10.1111/jcmm.14265
Anotace	We report results showing that the silencing of carbonic anhydrase I (siCA1) in prostatic (PC3) tumour cells has a significant impact on exosome formation. An increased diameter, concentration and diversity of the produced exosomes were noticed as a consequence of this knock-down. The protein composition of the exosomes' cargo was also altered. Liquid chromatography and mass spectrometry analyses identified 42 proteins significantly altered in PC3 siCA1 exosomes compared with controls. The affected proteins are mainly involved in metabolic processes, biogenesis, cell component organization and defense/immunity. Interestingly, almost all of them have been described as 'enhancers' of tumour development through the promotion of cell proliferation, migration and invasion. Thus, our results indicate that the reduced expression of the CA1 protein enhances the malignant potential of PC3 cells.
Pracoviště	Mikrobiologický ústav
Kontakt	Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231
Rok sběru	2020
Elektronická adresa	https://onlinelibrary.wiley.com/doi/full/10.1111/jcmm.14265

Počet záznamů: 1