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Ecotoxicology impact of silica-coated CdSe/ZnS quantum dots internalized in Chlamydomonas reinhardtii algal cells

SYSNO ASEP	0508300
Druh ASEP	J - Článek v odborném periodiku
Zařazení RIV	J - Článek v odborném periodiku
Poddruh J	Článek ve WOS
Název	Ecotoxicology impact of silica-coated CdSe/ZnS quantum dots internalized in Chlamydomonas reinhardtii algal cells
Tvůrce(i)	Elzorkany, H.E. (EG) Farghali, M.A. (EG) Hassan, M. A. (EG) El-sayed, Kh. (EG) Canonico, Myriam (MBU-M)_ORCID Konert, Grzegorz (MBU-M)_ORCID Farroh, K. (EG) Elshoky, H.A. (EG) Kaňa, Radek (MBU-M)_{RID, ORCID}
Zdroj.dok.	Science of the Total Environment. - : Elsevier - ISSN 0048-9697 Roč. 666, MAY 20 (2019), s. 480-489
Poč.str.	10 s.
Jazyk dok.	eng - angličtina
Země vyd.	NL - Nizozemsko
Klíč. slova	Photosynthetic organisms ; Nanocrystals ; Confocal microscopy
Vědní obor RIV	EH - Ekologie - společenstva
Obor OECD	Environmental sciences (social aspects to be 5.7)
CEP	GA16-10088S GA ČR - Grantová agentura ČR
	LO1416 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy
	ED2.1.00/19.0392 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy
Způsob publikování	Omezený přístup
Institucionální podpora	MBU-M - RVO:61388971
UT WOS	000463180700047
EID SCOPUS	85061824966
DOI	10.1016/j.scitotenv.2019.02.274
Anotace	The use of quantum dots (QD) in various medical and industrial applications may cause these nanoparticles to leak into waterways and subsequently enter the food chain. Therefore, if we intend to use QD, we must first know their potential environmental implications. In this work, cadmium selenide/zinc sulfide core/shell QD were synthesized, and then, biocompatible, water-dispersed QD were coated with silica (Si-QD). The QD were characterized by X-ray diffraction (XRD), high-resolution transmission electron microscopy (HRTEM) combined with energy-dispersive X-ray spectroscopy (EDX), and UV-Vis absorption analysis, which revealed that these surface-engineered QD have a highly crystalline, homogeneous spherical shape measuring approximately 25 nm. The cytotoxicity of the nanoparticles in the green algae Chlamydomonas reinhardtii was studied by incubating the algae cells with Si-QD and determining the optical density of algal cell culture, cell counts, and cells sizes by microflow cytometry. These measurements indicated that Si-QD are biocompatible up to a concentration of 25 ng/ml. Finally, the cellular uptake of Si-QD into C. reinhardtii was monitored by confocal laser scanning microscopy (CLSM). In conclusion, our results reveal that surface-engineered Cd-QD can penetrate the cells of aquatic organisms, which ensures a serious impact on the food chain and consequently the environment. On the other hand, the results also highlight a new potential method for bioremediation of Cd-QD by green algae, especially C. reinhardtii.
Pracoviště	Mikrobiologický ústav
Kontakt	Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231
Rok sběru	2020
Elektronická adresa	https://www.sciencedirect.com/science/article/pii/S0048969719307727?via%3Dihub

Počet záznamů: 1