Počet záznamů: 1
Unique Gene Expression Signatures in the Intestinal Mucosa and Organoids Derived from Germ-Free and Monoassociated Mice
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SYSNO ASEP 0508126 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Unique Gene Expression Signatures in the Intestinal Mucosa and Organoids Derived from Germ-Free and Monoassociated Mice Tvůrce(i) Janečková, L. (CZ)
Kostovčíková, Klára (MBU-M) ORCID
Švec, J. (CZ)
Šťastná, M. (CZ)
Strnad, H. (CZ)
Kolář, M. (CZ)
Hudcovic, Tomáš (MBU-M) RID, ORCID
Stančíková, J. (CZ)
Turečková, J. (CZ)
Baloghová, N. (CZ)
Sloncová, E. (CZ)
Galušková, K. (CZ)
Tlaskalová-Hogenová, Helena (MBU-M) RID, ORCID
Kořínek, V. (CZ)Číslo článku 1581 Zdroj.dok. International Journal of Molecular Sciences. - : MDPI
Roč. 20, č. 7 (2019)Poč.str. 20 s. Jazyk dok. eng - angličtina Země vyd. CH - Švýcarsko Klíč. slova expression profiling ; Enricher tool ; microbiota Vědní obor RIV EE - Mikrobiologie, virologie Obor OECD Microbiology CEP GA16-06326S GA ČR - Grantová agentura ČR GA19-08294S GA ČR - Grantová agentura ČR Způsob publikování Open access Institucionální podpora MBU-M - RVO:61388971 UT WOS 000465258100012 EID SCOPUS 85064201583 DOI 10.3390/ijms20071581 Anotace Commensal microbiota contribute to gut homeostasis by inducing transcription of mucosal genes. Analysis of the impact of various microbiota on intestinal tissue provides an important insight into the function of this organ. We used cDNA microarrays to determine the gene expression signature of mucosa isolated from the small intestine and colon of germ-free (GF) mice and animals monoassociated with two E. coli strains. The results were compared to the expression data obtained in conventionally reared (CR) mice. In addition, we analyzed gene expression in colon organoids derived from CR, GF, and monoassociated animals. The analysis revealed that the complete absence of intestinal microbiota mainly affected the mucosal immune system, which was not restored upon monoassociation. The most important expression changes observed in the colon mucosa indicated alterations in adipose tissue and lipid metabolism. In the comparison of differentially expressed genes in the mucosa or organoids obtained from GF and CR mice, only six genes were common for both types of samples. The results show that the increased expression of the angiopoietin-like 4 (Angptl4) gene encoding a secreted regulator of lipid metabolism indicates the GF status. Pracoviště Mikrobiologický ústav Kontakt Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Rok sběru 2020 Elektronická adresa https://www.mdpi.com/1422-0067/20/7/1581
Počet záznamů: 1