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Purification of lutein from the green microalgae Chlorella vulgaris by integrated use of a new extraction protocol and a multi-injection high performance counter-current chromatography (HPCCC)
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SYSNO ASEP 0507964 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Purification of lutein from the green microalgae Chlorella vulgaris by integrated use of a new extraction protocol and a multi-injection high performance counter-current chromatography (HPCCC) Tvůrce(i) Fábryová, Tereza (MBU-M) ORCID
Cheel, José (MBU-M) RID, ORCID
Kubáč, David (MBU-M) RID, ORCID
Hrouzek, Pavel (MBU-M) ORCID
Vu, Dai Long (MBU-M) ORCID
Tůmová, Lenka (MBU-M)Číslo článku 101574 Zdroj.dok. Algal Research-Biomass Biofuels and Bioproducts. - : Elsevier - ISSN 2211-9264
Roč. 41, AUG 2019 (2019)Poč.str. 10 s. Jazyk dok. eng - angličtina Země vyd. NL - Nizozemsko Klíč. slova Lutein ; Microalgae ; Chlorella vulgaris Vědní obor RIV EE - Mikrobiologie, virologie Obor OECD Microbiology CEP LO1416 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy FV10155 GA MPO - Ministerstvo průmyslu a obchodu Způsob publikování Omezený přístup Institucionální podpora MBU-M - RVO:61388971 UT WOS 000472593800041 EID SCOPUS 85067057566 DOI 10.1016/j.algal.2019.101574 Anotace Lutein is a natural carotenoid with health promoting properties whose commercial source is the yellow Marigold flower, but its production is limited by climate, planting area and high labor costs. The microalga Chlorella vulgaris is a promising lutein source however, no commercial lutein production uses microalgae. In the present study, the lower phase (LP4) of the biphasic solvent system composed of n-heptane-ethanol-water (5:4:1.5, v/v/v) was used both as a solvent for microalgae biomass extraction and as a mobile phase for lutein isolation using high performance counter-current chromatography (HPCCC). The ultrasound-assisted extraction of biomass with LP4 for 30 min led to an extract with a lutein content (3.20 mg/g dried biomass) that was higher than those obtained by the reference solvents tetrahydrofuran and dichloromethane. A multi-injection HPCCC method was developed for lutein isolation, where LP4 was pumped as the mobile phase at a flow rate of 8 mL/min, whereas its upper phase was the stationary phase. In total, 2 g of C. vulgaris extract were processed affording 60 mg of lutein (92% purity), which was further cleaned up by gel permeation chromatography yielding 50 mg of lutein (97% purity). The chemical identity of the purified compound was confirmed by mass spectrometry and absorption spectroscopy in comparison with an authentic standard. The developed HPCCC method may serve as a reference for promoting the development of a new production model of microalgae-based lutein. Pracoviště Mikrobiologický ústav Kontakt Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Rok sběru 2020 Elektronická adresa https://www.sciencedirect.com/science/article/pii/S2211926419303418?via%3Dihub
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