Počet záznamů: 1
Chemoenzymatic Synthesis and Radical Scavenging of Sulfated Hydroxytyrosol, Tyrosol, and Acetylated Derivatives
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SYSNO ASEP 0507895 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Chemoenzymatic Synthesis and Radical Scavenging of Sulfated Hydroxytyrosol, Tyrosol, and Acetylated Derivatives Tvůrce(i) Begines, P. (ES)
Biedermann, David (MBU-M) RID, ORCID
Valentová, Kateřina (MBU-M) RID, ORCID
Petrásková, Lucie (MBU-M) ORCID
Pelantová, Helena (MBU-M) ORCID, RID
Maya, I. (ES)
Fernandez-Bolanos, J.G. (ES)
Křen, Vladimír (MBU-M) RID, ORCIDZdroj.dok. Journal of Agricultural and Food Chemistry. - : American Chemical Society - ISSN 0021-8561
Roč. 67, č. 26 (2019), s. 7281-7288Poč.str. 8 s. Jazyk dok. eng - angličtina Země vyd. US - Spojené státy americké Klíč. slova olive phenols ; arylsulfotransferase ; hydroxytyrosol Vědní obor RIV CE - Biochemie Obor OECD Biochemistry and molecular biology CEP GA18-00121S GA ČR - Grantová agentura ČR GA19-00043S GA ČR - Grantová agentura ČR Způsob publikování Omezený přístup Institucionální podpora MBU-M - RVO:61388971 UT WOS 000474669600009 EID SCOPUS 85069265451 DOI 10.1021/acs.jafc.9b01065 Anotace Potential metabolites of bioactive compounds are important for their biological activities and as authentic standards for metabolic studies. The phenolic compounds contained in olive oil are an important part of the human diet, and therefore their potential metabolites are of utmost interest. We developed a convenient, scalable, one-pot chemoenzymatic method using the arylsulfotransferase from Desulfitobacterium hafniense for the sulfation of the natural olive oil phenols tyrosol, hydroxytyrosol, and of their monoacetylated derivatives. Respective monosulfated (tentative) metabolites were fully structurally characterized using LC-MS, NMR, and HRMS. In addition, Folin-Ciocalteu reduction, 1,1-diphenyl-2-picrylhydrazyl radical scavenging, and antilipoperoxidant activity in rat liver microsomes damaged by tert-butylhydroperoxide were measured and compared to the parent compounds. As expected, the sulfation diminished the radical scavenging properties of the prepared compounds. These compounds will serve as authentic standards of phase II metabolites. Pracoviště Mikrobiologický ústav Kontakt Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Rok sběru 2020 Elektronická adresa https://pubs.acs.org/doi/10.1021/acs.jafc.9b01065
Počet záznamů: 1