Počet záznamů: 1
Identification of Coxiella burnetii surface-exposed and cell envelope associated proteins using a combined bioinformatics plus proteomics strategy
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SYSNO ASEP 0506709 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Identification of Coxiella burnetii surface-exposed and cell envelope associated proteins using a combined bioinformatics plus proteomics strategy Tvůrce(i) Flores-Ramirez, G. (SK)
Jankovičová, B. (SK)
Bílková, Z. (SK)
Miernyk, J.A. (US)
Škultéty, L'udovít (MBU-M) ORCID, RIDZdroj.dok. Proteomics. - : Wiley - ISSN 1615-9853
Roč. 14, č. 16 (2014), s. 1868-1881Poč.str. 14 s. Jazyk dok. eng - angličtina Země vyd. US - Spojené státy americké Klíč. slova Bioinformatic predictions ; Coxiella burnetii ; Microbiology Vědní obor RIV EE - Mikrobiologie, virologie Obor OECD Microbiology Způsob publikování Omezený přístup Institucionální podpora MBU-M - RVO:61388971 UT WOS 000340422000003 EID SCOPUS 84905715131 DOI 10.1002/pmic.201300338 Anotace The Gram-negative pathogen Coxiella burnetii is an intracellular bacterium that replicates within the phagolysosomal vacuoles of eukaryotic cells. This pathogen can infect a wide range of hosts, and is the causative agent of Q fever in humans. Surface-exposed and cell envelope associated proteins are thought to be important for both pathogenesis and protective immunity. Herein, we propose a complementary strategy consisting of (i) in silico prediction and (ii) inventory of the proteomic composition using three enrichment approaches coupled with protein identification. The efficiency of classical Triton X-114 phase partitioning was compared with two novel procedures isolation of alkaline proteins by liquid-phase IEF, and cell surface enzymatic shaving using biofunctional magnetic beads. Of the 2026 protein sequences analyzed using seven distinct bioinformatic algorithms, 157 were predicted to be outer membrane proteins (OMP) and/or lipoproteins (LP). Using the three enrichment protocols, we identified 196 nonredundant proteins, including 39 predicted OMP and/or LP, 32 unknown or poorly characterized proteins, and 17 effectors of the Type IV secretion system. We additionally identified eight proteins with moonlighting activities, and several proteins apparently peripherally associated with integral or anchored OMP and/or LP. Pracoviště Mikrobiologický ústav Kontakt Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Rok sběru 2020 Elektronická adresa https://onlinelibrary.wiley.com/doi/abs/10.1002/pmic.201300338
Počet záznamů: 1