Počet záznamů: 1  

Identification of Coxiella burnetii surface-exposed and cell envelope associated proteins using a combined bioinformatics plus proteomics strategy

  1. 1.
    SYSNO ASEP0506709
    Druh ASEPJ - Článek v odborném periodiku
    Zařazení RIVJ - Článek v odborném periodiku
    Poddruh JČlánek ve WOS
    NázevIdentification of Coxiella burnetii surface-exposed and cell envelope associated proteins using a combined bioinformatics plus proteomics strategy
    Tvůrce(i) Flores-Ramirez, G. (SK)
    Jankovičová, B. (SK)
    Bílková, Z. (SK)
    Miernyk, J.A. (US)
    Škultéty, L'udovít (MBU-M) ORCID, RID
    Zdroj.dok.Proteomics. - : Wiley - ISSN 1615-9853
    Roč. 14, č. 16 (2014), s. 1868-1881
    Poč.str.14 s.
    Jazyk dok.eng - angličtina
    Země vyd.US - Spojené státy americké
    Klíč. slovaBioinformatic predictions ; Coxiella burnetii ; Microbiology
    Vědní obor RIVEE - Mikrobiologie, virologie
    Obor OECDMicrobiology
    Způsob publikováníOmezený přístup
    Institucionální podporaMBU-M - RVO:61388971
    UT WOS000340422000003
    EID SCOPUS84905715131
    DOI10.1002/pmic.201300338
    AnotaceThe Gram-negative pathogen Coxiella burnetii is an intracellular bacterium that replicates within the phagolysosomal vacuoles of eukaryotic cells. This pathogen can infect a wide range of hosts, and is the causative agent of Q fever in humans. Surface-exposed and cell envelope associated proteins are thought to be important for both pathogenesis and protective immunity. Herein, we propose a complementary strategy consisting of (i) in silico prediction and (ii) inventory of the proteomic composition using three enrichment approaches coupled with protein identification. The efficiency of classical Triton X-114 phase partitioning was compared with two novel procedures isolation of alkaline proteins by liquid-phase IEF, and cell surface enzymatic shaving using biofunctional magnetic beads. Of the 2026 protein sequences analyzed using seven distinct bioinformatic algorithms, 157 were predicted to be outer membrane proteins (OMP) and/or lipoproteins (LP). Using the three enrichment protocols, we identified 196 nonredundant proteins, including 39 predicted OMP and/or LP, 32 unknown or poorly characterized proteins, and 17 effectors of the Type IV secretion system. We additionally identified eight proteins with moonlighting activities, and several proteins apparently peripherally associated with integral or anchored OMP and/or LP.
    PracovištěMikrobiologický ústav
    KontaktEliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231
    Rok sběru2020
    Elektronická adresahttps://onlinelibrary.wiley.com/doi/abs/10.1002/pmic.201300338
Počet záznamů: 1  

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