Počet záznamů: 1
EFFECT OF BONE MORPHOGENETIC PROTEIN-15 ON GONADOTROPIN-STIMULATED SYNTHESIS OF HYALURONAN AND PROGESTERONE IN PORCINE OVARIAN FOLLICLE
- 1.
SYSNO ASEP 0506398 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název EFFECT OF BONE MORPHOGENETIC PROTEIN-15 ON GONADOTROPIN-STIMULATED SYNTHESIS OF HYALURONAN AND PROGESTERONE IN PORCINE OVARIAN FOLLICLE Tvůrce(i) Nagyová, Eva (UZFG-Y) RID, ORCID
Němcová, Lucie (UZFG-Y) RID
Bujnaková Mlynarčíková, A. (SK)
Blaha, Milan (UZFG-Y) RID
Procházka, Radek (UZFG-Y) RID, ORCID
Scsuková, S. (SK)Zdroj.dok. Journal of Physiology and Pharmacology. - : Polskie Towarzystwo Fizjologiczne - ISSN 0867-5910
Roč. 68, č. 5 (2017), s. 683-691Poč.str. 9 s. Forma vydání Tištěná - P Jazyk dok. eng - angličtina Země vyd. PL - Polsko Klíč. slova bone morphogenetic protein-15 ; cumulus extracellular matrix ; granulosa cells Vědní obor RIV EB - Genetika a molekulární biologie Obor OECD Developmental biology CEP EF15_003/0000460 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Způsob publikování Omezený přístup Institucionální podpora UZFG-Y - RVO:67985904 UT WOS 000436909100004 EID SCOPUS 85041729765 Anotace Bone morphogenetic protein-15 (BMP-15), an oocyte-derived growth factor, has been shown to play integral roles in regulation of ovarian follicular function in mammals. Despite the recognition of the physiological importance of the BMP system in regulation of gonadotropin action in the ovary, molecular mechanisms of BMP-15 effect on oocyte and somatic follicular cell functions remain poorly understood. The objective of this study was to determine the effect of BMP-15 on the FSH/LH-stimulated synthesis of hyaluronan (HA) by oocyte annulus complexes (OCC) and progesterone by OCC and granulosa cells (GC) in the presence or absence of serum using primary porcine cultures. In addition, the effect of BMP-15 on oocyte maturation- and steroidogenesis-related transcripts after 4, 8, 16, and 24 hours of cultivation was evaluated using real-time RT-PCR. We demonstrated that the FSH/LH-induced cumulus expansion was accompanied by a significant increase in CD44, PTGS2, CYP11A1 (at 4 h) and AREG, HAS2, TIVFAIP6, STAR (at 8 h) mRNAs. While FSH/LH-stimulated total HA synthesis by OCC was not affected by BMP-15 in serum-supplemented medium, its retention within the complex was significantly increased after the action of BMP-15 in comparison to FSH/LH alone (P < 0.001, 65% versus 35%. respectively). Moreover, we detected a significant increase in the expression of AREG and TNFAIP6 (both at 16 h), and CYPIIA1 (at 24 h) in FSH/LH-stimulated OCC due to the action of BMP-15 compared to complexes cultured only with FSH/LH. In the presence of serum, BMP-15 markedly increased FSH/LH-stimulated progesterone secretion by OCC (about 69%) and induced a significant decrease in FSH/LH-induced progesterone release by GC (about 35%) compared to FSH/LH alone. The present results indicate that the addition of BMP-15 to the gonadotropin-stimulated OCC cultured in serum-supplemented medium might improve oocyte-cumulus maturation. Pracoviště Ústav živočišné fyziologie a genetiky Kontakt Jana Zásmětová, knihovna@iapg.cas.cz, Tel.: 315 639 554 Rok sběru 2020 Elektronická adresa https://asep.lib.cas.cz/arl-cav/cs/csg/?repo=crepo1&key=11137804280
Počet záznamů: 1