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Anaerobic fungal communities differ along the horse digestive tract
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SYSNO ASEP 0503750 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Anaerobic fungal communities differ along the horse digestive tract Tvůrce(i) Mura, E. (IT)
Edwards, B. (NL)
Kittelmann, S. (DE)
Kaerger, K. (DE)
Voigt, K. (DE)
Mrázek, Jakub (UZFG-Y) RID, ORCID
Moniello, G. (IT)
Fliegerová, Kateřina (UZFG-Y) RID, ORCIDZdroj.dok. Fungal Biology. - : Elsevier - ISSN 1878-6146
Roč. 123, č. 3 (2019), s. 240-246Poč.str. 7 s. Forma vydání Tištěná - P Jazyk dok. eng - angličtina Země vyd. GB - Velká Británie Klíč. slova anaerobic fungi ; diversity ; equine hindgut Vědní obor RIV EE - Mikrobiologie, virologie Obor OECD Microbiology CEP EF15_003/0000460 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Způsob publikování Omezený přístup Institucionální podpora UZFG-Y - RVO:67985904 UT WOS 000461403100007 EID SCOPUS 85059307557 DOI 10.1016/j.funbio.2018.12.004 Anotace Anaerobic fungi are potent fibre degrading microbes in the equine hindgut, yet our understanding of their diversity and community structure is limited to date. In this preliminary work, using a clone library approach we studied the diversity of anaerobic fungi along six segments of the horse hindgut: caecum, right ventral colon (RVC), left ventral colon (LVC), left dorsal colon (LDC), right dorsal colon (RDC) and rectum. Of the 647 ITS1 clones, 61.7 % were assigned to genus level groups that are so far without any cultured representatives, and 38.0 % were assigned to the cultivated genera Neocallimastix (35.1 %), Orpinomyces (2.3 %), and Anaeromyces (0.6 %). AL1 dominated the group of uncultured anaerobic fungi, particularly in the RVC (88 %) and LDC (97 %). Sequences from the LSU clone library analysis of the LDC, however, split into two distinct phylogenetic clusters with low sequence identity to Caecomyces sp. (94-96 %) and Liebetanzomyces sp. (92 %) respectively. Sequences belonging to cultured Neocallimastix spp. dominated in LVC (81 %) and rectum (75.5 %). Quantification of anaerobic fungi showed significantly higher concentrations in RVC and RDC compared to other segments, which influenced the interpretation of the changes in anaerobic fungal diversity along the horse hindgut. These preliminary findings require further investigation. Pracoviště Ústav živočišné fyziologie a genetiky Kontakt Jana Zásmětová, knihovna@iapg.cas.cz, Tel.: 315 639 554 Rok sběru 2020 Elektronická adresa https://asep.lib.cas.cz/arl-cav/cs/csg/?repo=crepo1&key=13449112003
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