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Nanodiamonds as Effective Vectors for Micro RNA-Based Drug Delivery
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SYSNO ASEP 0499815 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Ostatní články Název Nanodiamonds as Effective Vectors for Micro RNA-Based Drug Delivery Tvůrce(i) Benson, Veronika (MBU-M) RID, ORCID
Neuhoferová, Eva (MBU-M) ORCIDZdroj.dok. Drug Designing & Intellectual Properties International Journal. - : Lupine Publishers - ISSN 2637-4706
Roč. 1, č. 4 (2018), s. 112-117Poč.str. 6 s. Jazyk dok. eng - angličtina Země vyd. US - Spojené státy americké Klíč. slova Micro-RNA ; RNA interference ; Nano Diamond Vědní obor RIV EE - Mikrobiologie, virologie Obor OECD Microbiology CEP NV15-33094A GA MZd - Ministerstvo zdravotnictví Institucionální podpora MBU-M - RVO:61388971 Anotace Micro RNAs are short non-coding RNA molecules, which are important regulators of cell functions. Deregulation of certain micro RNA level is contributing factor to tumor formation and progression. Possible gene therapy by modulation of the micro RNA level is currently considered. Recently it has been proved that nano diamonds coated with PEI serve as efficient transfection and monitoring system. The aim of our work was to deliver micro RNA inhibitor and micro RNA mimic sequences into specialized mammalian
cells using fluorescent nano diamond particles and to test the nano complex uptake and cell compatibility during primary in vivo application. Short RNA molecules were bound to the PEI coated nano diamonds, transported into the colon cancer cell line in vitro and observed in a confocal microscope. The miRNA mimic coated nano diamonds were used for complex uptake and toxicity study in peritoneal macrophages and splenocytes after ex vivo stimulation or in vivo administration. Due to the possibility of continuous
monitoring of carrier internalization, its transfection efficacy, and cell compatibility, the fluorescent nano diamonds represent a suitable RNA carrier with a potential use in gene therapy.Pracoviště Mikrobiologický ústav Kontakt Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Rok sběru 2019
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