Počet záznamů: 1
The intracellular distribution of inorganic carbon fixing enzymes does not support the presence of a C4 pathway in the diatom Phaeodactylum tricornutum
- 1.
SYSNO ASEP 0499451 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název The intracellular distribution of inorganic carbon fixing enzymes does not support the presence of a C4 pathway in the diatom Phaeodactylum tricornutum Tvůrce(i) Ewe, Daniela (MBU-M) ORCID
Tachibana, M. (JP)
Kikutani, S. (JP)
Gruber, Ansgar (BC-A) RID, ORCID
Bártulos, C.R. (DE)
Konert, Grzegorz (MBU-M) ORCID
Kaplan, A. (IL)
Matsuda, Y. (JP)
Kroth, P. G. (DE)Zdroj.dok. Photosynthesis Research. - : Springer - ISSN 0166-8595
Roč. 137, č. 2 (2018), s. 263-280Poč.str. 18 s. Jazyk dok. eng - angličtina Země vyd. NL - Nizozemsko Klíč. slova C4 photosynthesis ; Chloroplast ; Green fluorescent protein (GFP) Vědní obor RIV EF - Botanika Obor OECD Plant sciences, botany Vědní obor RIV – spolupráce Biologické centrum (od r. 2006) - Botanika Institucionální podpora MBU-M - RVO:61388971 ; BC-A - RVO:60077344 UT WOS 000437397700011 EID SCOPUS 85044386198 DOI 10.1007/s11120-018-0500-5 Anotace Diatoms are unicellular algae and important primary producers. The process of carbon fixation in diatoms is very efficient even though the availability of dissolved CO2 in sea water is very low. The operation of a carbon concentrating mechanism (CCM) also makes the more abundant bicarbonate accessible for photosynthetic carbon fixation. Diatoms possess carbonic anhydrases as well as metabolic enzymes potentially involved in C4 pathways, however, the question as to whether a C4 pathway plays a general role in diatoms is not yet solved. While genome analyses indicate that the diatom Phaeodactylum tricornutum possesses all the enzymes required to operate a C4 pathway, silencing of the pyruvate orthophosphate dikinase (PPDK) in a genetically transformed cell line does not lead to reduced photosynthetic carbon fixation. In this study, we have determined the intracellular location of all enzymes potentially involved in C4-like carbon fixing pathways in P. tricornutum by expression of the respective proteins fused to green fluorescent protein (GFP), followed by fluorescence microscopy. Furthermore, we compared the results to known pathways and locations of enzymes in higher plants performing C3 or C4 photosynthesis. This approach revealed that the intracellular distribution of the investigated enzymes is quite different from the one observed in higher plants. In particular, the apparent lack of a plastidic decarboxylase in P. tricornutum indicates that this diatom does not perform a C4-like CCM. Pracoviště Mikrobiologický ústav Kontakt Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Rok sběru 2019
Počet záznamů: 1