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Nanodiamonds as an innovative system for intracellular delivery of mirna-34a inprostatic cancer therapy
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SYSNO ASEP 0499421 Druh ASEP C - Konferenční příspěvek (mezinárodní konf.) Zařazení RIV D - Článek ve sborníku Název Nanodiamonds as an innovative system for intracellular delivery of mirna-34a inprostatic cancer therapy Tvůrce(i) Bitti, G. (IT)
Abate, M. (IT)
Neuhoferová, Eva (MBU-M) ORCID
Kindermann, Marek (MBU-M)
Petráková, V. (CZ)
Boccellino, M. (IT)
Quagliuolo, L. (IT)
Filová, Eva (UEM-P) RID, ORCID
Benson, Veronika (MBU-M) RID, ORCID
Caraglia, M. (IT)
Amler, Evžen (UEM-P) RIDZdroj.dok. Nanodiamonds as an innovative system for intracellular delivery of mirna-34a inprostatic cancer therapy. - Ostrava : Tanger Ltd, 2018 - ISBN 978-80-87294-81-9 Rozsah stran s. 449-454 Poč.str. 6 s. Forma vydání Tištěná - P Akce 9th International Conference on Nanomaterials - Research and Application (NANOCON) Datum konání 18.10.2017 - 20.10.2017 Místo konání Brno Země CZ - Česká republika Typ akce WRD Jazyk dok. eng - angličtina Země vyd. CZ - Česká republika Klíč. slova miR-34a ; nanodiamonds ; prostate cancer Vědní obor RIV EE - Mikrobiologie, virologie Obor OECD Microbiology CEP NV15-33094A GA MZd - Ministerstvo zdravotnictví Institucionální podpora MBU-M - RVO:61388971 ; UEM-P - RVO:68378041 UT WOS 000452823300073 EID SCOPUS 85052292166 Anotace The microRNA(miRNA)-34a is an important regulator of tumor suppression. It controls the expression of several target proteins involved in cell cycle, differentiation and apoptosis, and antagonizes processes that are necessary for basic cancer cell viability as well as cancer stemness, metastasis, and chemoresistance. It is downregulated in numerous cancer types, including prostatic cancer, and inhibits malignant growth by repressing genes involved in various oncogenic signaling pathways. Given the anti-oncogenic activity of miR-34a, here we proved the substantial benefits of a new therapeutic concept based on nanotechnology delivery of miRNA mimics. In order to monitor the miRNA-34a replacement, we used a fluorescent nanodiamond particles (FND) system with linked miRNA-34a mimic, which was delivered to PC3 and DU145 prostatic cancer cell lines. We used functionalized nanodiamonds coated with polyethylenimine to transfer miRNA-34a into PC3 and DU145 prostatic cancer cell lines and we measured the zeta-potential of these complexes before using them for in vitro experiments. A replacement of miRNA-34 was observed by monitoring levels of miRNA-34 via real-time PCR. Moreover, our in vitro experiments demonstrated that miRNA-34a replacement, using this FND delivery system, decreased viability and induced apoptosis in prostatic cancer cell lines. Our findings suggest the replacement of oncosuppressor miRNA-34a provides an effective strategy for cancer therapy and the FND-based delivery systems seems to be an excellent strategy for a safe and effective targeting of the tumor. Pracoviště Mikrobiologický ústav Kontakt Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Rok sběru 2019
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