Počet záznamů: 1

Orientation of Laurdan in Phospholipid Bilayers Influences Its Fluorescence: Quantum Mechanics and Classical Molecular Dynamics Study

SYSNO ASEP	0499044
Druh ASEP	J - Článek v odborném periodiku
Zařazení RIV	J - Článek v odborném periodiku
Poddruh J	Článek ve WOS
Název	Orientation of Laurdan in Phospholipid Bilayers Influences Its Fluorescence: Quantum Mechanics and Classical Molecular Dynamics Study
Tvůrce(i)	Baig, Mirza Wasif (UFCH-W) Pederzoli, Marek (UFCH-W)_{ORCID, RID} Jurkiewicz, Piotr (UFCH-W)_{RID, ORCID} Cwiklik, Lukasz (UFCH-W)_{RID, ORCID} Pittner, Jiří (UFCH-W)_{RID, ORCID}
Číslo článku	1707
Zdroj.dok.	Molecules. - : MDPI Roč. 23, č. 7 (2018)
Poč.str.	12 s.
Jazyk dok.	eng - angličtina
Země vyd.	CH - Švýcarsko
Klíč. slova	water ; simulation ; vesicles ; program ; prodan ; model ; fluorescence
Vědní obor RIV	CF - Fyzikální chemie a teoretická chemie
Obor OECD	Physical chemistry
CEP	GAP208/12/0559 GA ČR - Grantová agentura ČR
	GA18-26751S GA ČR - Grantová agentura ČR
Způsob publikování	Open access
Institucionální podpora	UFCH-W - RVO:61388955
UT WOS	000445301800210
EID SCOPUS	85055604541
DOI	10.3390/molecules23071707
Anotace	Fluidity of lipid membranes is known to play an important role in the functioning of living organisms. The fluorescent probe Laurdan embedded in a lipid membrane is typically used to assess the fluidity state of lipid bilayers by utilizing the sensitivity of Laurdan emission to the properties of its lipid environment. In particular, Laurdan fluorescence is sensitive to gel vs liquid-crystalline phases of lipids, which is demonstrated in different emission of the dye in these two phases. Still, the exact mechanism of the environment effects on Laurdan emission is not understood. Herein, we utilize dipalmitoylphosphatidylcholine (DPPC) and dioleoylphosphatidylcholine (DOPC) lipid bilayers, which at room temperature represent gel and liquid-crystalline phases, respectively. We simulate absorption and emission spectra of Laurdan in both DOPC and DPPC bilayers with quantum chemical and classical molecular dynamics methods. We demonstrate that Laurdan is incorporated in heterogeneous fashion in both DOPC and DPPC bilayers, and that its fluorescence depends on the details of this embedding.
Pracoviště	Ústav fyzikální chemie J.Heyrovského
Kontakt	Michaela Knapová, michaela.knapova@jh-inst.cas.cz, Tel.: 266 053 196
Rok sběru	2019

Počet záznamů: 1