Počet záznamů: 1
Biocompatible glyconanomaterials based on HPMA-copolymer for specific targeting of galectin-3
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SYSNO ASEP 0496863 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Biocompatible glyconanomaterials based on HPMA-copolymer for specific targeting of galectin-3 Tvůrce(i) Bojarová, Pavla (MBU-M) ORCID
Tavares, Marina Rodrigues (UMCH-V) ORCID, RID
Laaf, D. (DE)
Bumba, Ladislav (MBU-M) RID, ORCID
Petrásková, Lucie (MBU-M) ORCID
Konefal, Rafal (UMCH-V) RID, ORCID
Bláhová, Markéta (UMCH-V) RID, ORCID
Pelantová, Helena (MBU-M) ORCID, RID
Elling, L. (DE)
Etrych, Tomáš (UMCH-V) RID, ORCID
Chytil, Petr (UMCH-V) RID, ORCID
Křen, Vladimír (MBU-M) RID, ORCIDČíslo článku 73 Zdroj.dok. Journal of Nanobiotechnology . - : BioMed Central
Roč. 16, SEP 20 (2018)Poč.str. 16 s. Jazyk dok. eng - angličtina Země vyd. GB - Velká Británie Klíč. slova Carbohydrate ; ELISA ; Galectin-3 Vědní obor RIV EI - Biotechnologie a bionika Obor OECD Microbiology Vědní obor RIV – spolupráce Ústav makromolekulární chemie - Makromolekulární chemie CEP GA17-13721S GA ČR - Grantová agentura ČR GA18-01163S GA ČR - Grantová agentura ČR NV16-28594A GA MZd - Ministerstvo zdravotnictví LO1507 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy LTC17005 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy LM2015064 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Institucionální podpora MBU-M - RVO:61388971 ; UMCH-V - RVO:61389013 UT WOS 000445211800001 EID SCOPUS 85055135018 DOI 10.1186/s12951-018-0399-1 Anotace Background: Galectin-3 (Gal-3) is a promising target in cancer therapy with a high therapeutic potential due to its abundant localization within the tumor tissue and its involvement in tumor development and proliferation. Potential clinical application of Gal-3-targeted inhibitors is often complicated by their insufficient selectivity or low biocompatibility. Nanomaterials based on N-(2-hydroxypropyl)methacrylamide (HPMA) nanocarrier are attractive for in vivo application due to their good water solubility and lack of toxicity and immunogenicity. Their conjugation with tailored carbohydrate ligands can yield specific glyconanomaterials applicable for targeting biomedicinally relevant lectins like Gal-3.
Results: In the present study we describe the synthesis and the structure-affinity relationship study of novel Gal-3-targeted glyconanomaterials, based on hydrophilic HPMA nanocarriers. HPMA nanocarriers decorated with varying amounts of Gal-3 specific epitope GaINA031,461cNAc (LacdiNAc) were analyzed in a competitive ELISA-type assay and their binding kinetics was described by surface plasmon resonance. We showed the impact of various linker types and epitope distribution on the binding affinity to Gal-3.The synthesis of specific functionalized LacdiNAc epitopes was accomplished under the catalysis by mutant beta-N-acetylhexosaminidases. The glycans were conjugated to statistic HPMA copolymer precursors through diverse linkers in a defined pattern and density using Cu(I)-catalyzed azide- alkyne cycloaddition. The resulting water-soluble and structurally flexible synthetic glyconanomaterials exhibited affinity to Gal-3 in low mu M range.
Conclusions: The results of this study reveal the relation between the linker structure, glycan distribution and the affinity of the glycopolymer nanomaterial to Gal-3.They pave the way to specific biomedicinal glyconanomaterials that target Gal-3 as a therapeutic goal in cancerogenesis and other disorders.Pracoviště Mikrobiologický ústav Kontakt Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Rok sběru 2019
Počet záznamů: 1