Počet záznamů: 1
Primary transcriptome analysis reveals importance of IS elements for the shaping of the transcriptional landscape of Bordetella pertussis
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SYSNO ASEP 0494878 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Primary transcriptome analysis reveals importance of IS elements for the shaping of the transcriptional landscape of Bordetella pertussis Tvůrce(i) Amman, F. (AT)
D'Halluin, A. (FR)
Antoine, R. (FR)
Huot, L. (FR)
Bíbová, Ilona (MBU-M) RID
Keidel, Kristina (MBU-M)
Slupek, S. (FR)
Bouquet, P. (FR)
Coutte, L. (FR)
Caboche, S. (FR)
Locht, C. (FR)
Večerek, Branislav (MBU-M) RID, ORCID
Hot, D. (FR)Zdroj.dok. RNA biology. - : Taylor & Francis - ISSN 1547-6286
Roč. 15, č. 7 (2018), s. 967-975Poč.str. 9 s. Jazyk dok. eng - angličtina Země vyd. US - Spojené státy americké Klíč. slova bordetella pertussis ; insertion sequence ; transcriptome Vědní obor RIV EE - Mikrobiologie, virologie Obor OECD Microbiology CEP GF16-34825L GA ČR - Grantová agentura ČR NV16-30782A GA MZd - Ministerstvo zdravotnictví EE2.3.20.0055 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy EE2.3.30.0003 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Institucionální podpora MBU-M - RVO:61388971 UT WOS 000445654400015 EID SCOPUS 85047119429 DOI 10.1080/15476286.2018.1462655 Anotace Bordetella pertussis is the causative agent of whooping cough, a respiratory disease still considered as a major public health threat and for which recent re-emergence has been observed. Constant reshuffling of Bordetella pertussis genome organization was observed during evolution. These rearrangements are essentially mediated by Insertion Sequences (IS), a mobile genetic elements present in more than 230 copies in the genome, which are supposed to be one of the driving forces enabling the pathogen to escape from vaccine-induced immunity.Here we use high-throughput sequencing approaches (RNA-seq and differential RNA-seq), to decipher Bordetella pertussis transcriptome characteristics and to evaluate the impact of IS elements on transcriptome architecture. Transcriptional organization was determined by identification of transcription start sites and revealed also a large variety of non-coding RNAs including sRNAs, leaderless mRNAs or long 3 and 5UTR including seven riboswitches. Unusual topological organizations, such as overlapping 5- or 3-extremities between oppositely orientated mRNA were also unveiled. The pivotal role of IS elements in the transcriptome architecture and their effect on the transcription of neighboring genes was examined. This effect is mediated by the introduction of IS harbored promoters or by emergence of hybrid promoters. This study revealed that in addition to their impact on genome rearrangements, most of the IS also impact on the expression of their flanking genes. Furthermore, the transcripts produced by IS are strain-specific due to the strain to strain variation in IS copy number and genomic context.
KeywordsPracoviště Mikrobiologický ústav Kontakt Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Rok sběru 2019
Počet záznamů: 1