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A Bordetella pertussis MgtC homolog plays a role in the intracellular survival
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SYSNO ASEP 0494094 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název A Bordetella pertussis MgtC homolog plays a role in the intracellular survival Tvůrce(i) Cafiero, J.H. (AR)
Lamberti, Y.A. (AR)
Surmann, K. (DE)
Večerek, Branislav (MBU-M) RID, ORCID
Rodriguez, E.M. (AR)Číslo článku e0203204 Zdroj.dok. PLoS ONE. - : Public Library of Science - ISSN 1932-6203
Roč. 13, č. 8 (2018)Poč.str. 17 s. Jazyk dok. eng - angličtina Země vyd. US - Spojené státy americké Klíč. slova HUMAN MACROPHAGES ; INTRAMACROPHAGE SURVIVAL ; EPITHELIAL-CELLS Vědní obor RIV EE - Mikrobiologie, virologie Obor OECD Microbiology CEP GF16-34825L GA ČR - Grantová agentura ČR Institucionální podpora MBU-M - RVO:61388971 UT WOS 000443388900090 EID SCOPUS 85052844111 DOI 10.1371/journal.pone.0203204 Anotace Bordetella pertussis, the causative agent of whooping cough, has the capability to survive inside the host cells. This process requires efficient adaptation of the pathogen to the intracellular environment and the associated stress. Among the proteins produced by the intracellular B. pertussis we identified a protein (BP0414) that shares homology with MgtC, a protein which was previously shown to be involved in the intracellular survival of other pathogens. To explore if BP0414 plays a role in B. pertussis intracellular survival a mutant strain defective in the production of this protein was constructed. Using standard in vitro growth conditions we found that BP0414 is required for B. pertussis growth under low magnesium availability or low pH, two environmental conditions that this pathogen might face within the host cell. Intracellular survival studies showed that MgtC is indeed involved in B. pertussis viability inside the macrophages. The use of bafilomycin A1, which inhibits phagosome acidification, abolished the survival defect of the mgtC deficient mutant strain suggesting that in intracellular B. pertussis the role of MgtC protein is mainly related to the bacterial adaptation to the acidic conditions found inside the of phagosomes. Overall, this work provides an insight into the importance of MgtC in B. pertussis pathogenesis and its contribution to bacterial survival within immune cells. Pracoviště Mikrobiologický ústav Kontakt Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Rok sběru 2019
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