Počet záznamů: 1  

Effects of pure plant secondary metabolites on methane production, rumen fermentation and rumen bacteria populations in vitro

  1. 1.
    SYSNO ASEP0492378
    Druh ASEPJ - Článek v odborném periodiku
    Zařazení RIVJ - Článek v odborném periodiku
    Poddruh JČlánek ve WOS
    NázevEffects of pure plant secondary metabolites on methane production, rumen fermentation and rumen bacteria populations in vitro
    Tvůrce(i) Joch, M. (CZ)
    Mrázek, Jakub (UZFG-Y) RID, ORCID
    Skřivanová, E. (CZ)
    Čermák, L. (CZ)
    Marounek, M. (CZ)
    Zdroj.dok.Journal of Animal Physiology and Animal Nutrition. - : Wiley - ISSN 0931-2439
    Roč. 102, č. 4 (2018), s. 869-881
    Poč.str.13 s.
    Forma vydáníTištěná - P
    Jazyk dok.eng - angličtina
    Země vyd.US - Spojené státy americké
    Klíč. slovaalkaloid ; essential oil ; Ion Torrent ; rumen bacteria ; rumen fermentation
    Vědní obor RIVEE - Mikrobiologie, virologie
    Obor OECDMicrobiology
    CEPGA16-12431S GA ČR - Grantová agentura ČR
    EF15_003/0000460 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy
    Institucionální podporaUZFG-Y - RVO:67985904
    UT WOS000438205400007
    EID SCOPUS85046014267
    DOI10.1111/jpn.12910
    AnotaceIn this study, the effects of seven pure plant secondary metabolites (PSMs) on rumen fermentation, methane (CH4) production and rumen bacterial community composition were determined. Two in vitro trials were conducted. In trial 1, nine concentrations of 8-hydroxyquinoline, -terpineol, camphor, bornyl acetate, -pinene, thymoquinone and thymol were incubated on separate days using in vitro 24-hr batch incubations. All compounds tested demonstrated the ability to alter rumen fermentation parameters and decrease CH4 production. However, effective concentrations differed among individual PSMs. The lowest concentrations that reduced (p<.05) CH4 production were as follows: 8mg/L of 8-hydroxyquinoline, 120mg/L of thymoquinone, 240mg/L of thymol and 480mg/L of -terpineol, camphor, bornyl acetate and -pinene. These concentrations were selected for use in trial 2. In trial 2, PSMs were incubated in one run. Methane was decreased (p<.05) by all PSMs at selected concentrations. Based on denaturing gradient gel electrophoresis analysis, different PSMs changed the composition of bacterial communities to different extents. As revealed by Ion Torrent sequencing, the effects of PSMs on relative abundance were most pronounced in the predominant families, especially in Lachnospiraceae, Succinivibrionaceae, Prevotellaceae, unclassified Clostridiales and Ruminococcaceae. The CH4 production was correlated negatively (-.72, p<.05) with relative abundance of Succinivibrionaceae and positively with relative abundance of Ruminococcaceae (.86, p<.05). In summary, this study identified three pure PSMs (8hydroxyquinoline, bornyl acetate and thymoquinone) with potentially promising effects on rumen CH4 production. The PSMs tested in this study demonstrated considerable impact on rumen bacterial communities even at the lowest concentrations that decreased CH4 production. The findings from this study may help to elucidate how PSMs affect rumen bacterial fermentation.
    PracovištěÚstav živočišné fyziologie a genetiky
    KontaktJana Zásmětová, knihovna@iapg.cas.cz, Tel.: 315 639 554
    Rok sběru2019
Počet záznamů: 1  

  Tyto stránky využívají soubory cookies, které usnadňují jejich prohlížení. Další informace o tom jak používáme cookies.