Počet záznamů: 1

Morphologically indistinguishable hybrid Carassius female with 156 chromosomes: A threat for the threatened crucian carp, C. carassius, L

SYSNO ASEP	0489488
Druh ASEP	J - Článek v odborném periodiku
Zařazení RIV	J - Článek v odborném periodiku
Poddruh J	Článek ve WOS
Název	Morphologically indistinguishable hybrid Carassius female with 156 chromosomes: A threat for the threatened crucian carp, C. carassius, L
Tvůrce(i)	Knytl, M. (CZ) Kalous, L. (CZ) Rylková, K. (CZ) Choleva, Lukáš (UZFG-Y)_{RID, ORCID} Merilä, J. (FI) Ráb, Petr (UZFG-Y)_{RID, ORCID}
Číslo článku	e0190924
Zdroj.dok.	PLoS ONE. - : Public Library of Science - ISSN 1932-6203 Roč. 13, č. 1 (2018)
Poč.str.	19 s.
Forma vydání	Online - E
Jazyk dok.	eng - angličtina
Země vyd.	US - Spojené státy americké
Klíč. slova	Carrasius carrasius ; chromosome
Vědní obor RIV	EB - Genetika a molekulární biologie
Obor OECD	Genetics and heredity (medical genetics to be 3)
CEP	EF15_003/0000460 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy
Institucionální podpora	UZFG-Y - RVO:67985904
UT WOS	000423161800011
EID SCOPUS	85040968724
DOI	10.1371/journal.pone.0190924
Anotace	The crucian carp Carassius carassius (Linnaeus, 1758), is native to many European freshwaters. Despite its wide distribution, the crucian carp is declining in both the number and sizes of populations across much of its range. Here we studied 30 individuals of a putative pure population from Helsinki, Finland. Despite clear external morphological features of C. carassius, an individual was of a higher ploidy level than the others. We therefore applied a set of molecular genetic (S7 nuclear and cytochrome b mitochondrial genes) and cytogenetic tools (sequential fluorescent 4', 6-diamidino-2-phenylindole [DAPI], Chromomycin A(3) [CMA(3)], C-banding and in situ hybridization [FISH] with both 5S and 28S ribosomal DNA probes) to determine its origin. While all examined characteristics of a diploid representative male (CCAHe2Fi) clearly corresponded to those of C. carassius, a triploid individual (CCAHe1Fi) was more complex. Phylogenetic analysis revealed that the nuclear genome of CCAHe1Fi contained three haploid sets: two C. gibelio and one C. carassius. However the mitochondrial DNA was that of C. gibelio, demonstrating its hybrid origin. The FISH revealed three strong (more intensive) 5S rDNA loci, confirming the triploid status, and an additional 24 weak (less intensive) signals were observed in the chromosome complement of CCAHe1Fi. On the other hand, only two strong and 16 weak 5S rDNA signals were visible on the chromosomes of the CCAHe2Fi male. 28S rDNA FISH revealed four strong signals in both CCAHe1Fi and CCAHe2Fi individuals. CMA(3) staining revealed four to six CMA(3)-positive bands of CCAHe1Fi, while that of diploids contained only two to four. The fact that a polyploid hybrid Carassius female with a strong invasive potential may share morphological characters typical for endangered C. carassius highlights a need to combine genetic investigations of Carassius cryptic diversity with conservation measures of C. carassius in Europe.
Pracoviště	Ústav živočišné fyziologie a genetiky
Kontakt	Jana Zásmětová, knihovna@iapg.cas.cz, Tel.: 315 639 554
Rok sběru	2019

Počet záznamů: 1