Počet záznamů: 1
Poly-N-Acetyllactosamine Neo-Glycoproteins as Nanomolar Ligands of Human Galectin-3: Binding Kinetics and Modeling
- 1.
SYSNO ASEP 0488926 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Poly-N-Acetyllactosamine Neo-Glycoproteins as Nanomolar Ligands of Human Galectin-3: Binding Kinetics and Modeling Tvůrce(i) Bumba, Ladislav (MBU-M) RID, ORCID
Laaf, D. (DE)
Spiwok, V. (CZ)
Elling, L. (DE)
Křen, Vladimír (MBU-M) RID, ORCID
Bojarová, Pavla (MBU-M) ORCIDČíslo článku 372 Zdroj.dok. International Journal of Molecular Sciences. - : MDPI
Roč. 19, č. 2 (2018)Poč.str. 17 s. Jazyk dok. eng - angličtina Země vyd. CH - Švýcarsko Klíč. slova carbohydrate ; galectin-3 ; galectins in diagnosis Vědní obor RIV CE - Biochemie Obor OECD Biochemistry and molecular biology CEP LTC17005 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy LM2015047 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy LM2015064 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy GC15-02578J GA ČR - Grantová agentura ČR Institucionální podpora MBU-M - RVO:61388971 UT WOS 000427527400056 EID SCOPUS 85041114916 DOI 10.3390/ijms19020372 Anotace Galectin-3 (Gal-3) is recognized as a prognostic marker in several cancer types. Its involvement in tumor development and proliferation makes this lectin a promising target for early cancer diagnosis and anti-cancer therapies. Gal-3 recognizes poly-N-acetyllactosamine (LacNAc)-based carbohydrate motifs of glycoproteins and glycolipids with a high specificity for internal LacNAc epitopes. This study analyzes the mode and kinetics of binding of Gal-3 to a series of multivalent neo-glycoproteins presenting complex poly-LacNAc-based oligosaccharide ligands on a scaffold of bovine serum albumin. These neo-glycoproteins rank among the strongest Gal-3 ligands reported, with K-d reaching sub-nanomolar values as determined by surface plasmon resonance. Significant differences in the binding kinetics were observed within the ligand series, showing the tetrasaccharide capped with N,N-diacetyllactosamine (LacdiNAc) as the strongest ligand of Gal-3 in this study. A molecular model of the Gal-3 carbohydrate recognition domain with docked oligosaccharide ligands is presented that shows the relations in the binding site at the molecular level. The neo-glycoproteins presented herein may be applied for selective recognition of Gal-3 both on the cell surface and in blood serum. Pracoviště Mikrobiologický ústav Kontakt Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Rok sběru 2019
Počet záznamů: 1