Počet záznamů: 1
Biomembrane Permeabilization: Statistics of Individual Leakage Events Harmonize the Interpretation of Vesicle Leakage
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SYSNO ASEP 0485588 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Biomembrane Permeabilization: Statistics of Individual Leakage Events Harmonize the Interpretation of Vesicle Leakage Tvůrce(i) Braun, S. (DE)
Pokorná, Šárka (UFCH-W) RID
Šachl, Radek (UFCH-W) RID, ORCID
Hof, Martin (UFCH-W) RID, ORCID
Heerklotz, H. (DE)
Hoernke, M. (DE)Zdroj.dok. ACS Nano. - : American Chemical Society - ISSN 1936-0851
Roč. 12, č. 1 (2018), s. 813-819Poč.str. 7 s. Jazyk dok. eng - angličtina Země vyd. US - Spojené státy americké Klíč. slova leakage ; dye release ; pore Vědní obor RIV CF - Fyzikální chemie a teoretická chemie Obor OECD Physical chemistry CEP GA17-03160S GA ČR - Grantová agentura ČR Institucionální podpora UFCH-W - RVO:61388955 UT WOS 000423495200087 EID SCOPUS 85042192020 DOI 10.1021/acsnano.7b08184 Anotace The mode of action of membrane-active molecules, such as
antimicrobial, anticancer, cell penetrating, and fusion peptides and their
synthetic mimics, transfection agents, drug permeation enhancers, and
biological signaling molecules (e.g., quorum sensing), involves either the
general or local destabilization of the target membrane or the formation
of defined, rather stable pores. Some effects aim at killing the cell, while
others need to be limited in space and time to avoid serious damage.
Biological tests reveal translocation of compounds and cell death but do
not provide a detailed, mechanistic, and quantitative understanding of the
modes of action and their molecular basis. Model membrane studies of
membrane leakage have been used for decades to tackle this issue, but
their interpretation in terms of biology has remained challenging and often quite limited. Here we compare two recent,
powerful protocols to study model membrane leakage: the microscopic detection of dye influx into giant liposomes and
time-correlated single photon counting experiments to characterize dye efflux from large unilamellar vesicles. A statistical
treatment of both data sets does not only harmonize apparent discrepancies but also makes us aware of principal issues that
have been confusing the interpretation of model membrane leakage data so far. Moreover, our study reveals a fundamental
difference between nano- and microscale systems that needs to be taken into account when conclusions about microscale
objects, such as cells, are drawn from nanoscale models.Pracoviště Ústav fyzikální chemie J.Heyrovského Kontakt Michaela Knapová, michaela.knapova@jh-inst.cas.cz, Tel.: 266 053 196 Rok sběru 2019
Počet záznamů: 1