Electrochemical determination of 2-nitrofluorene and investigation of its interaction with DNA
1.
SYSNO ASEP
0470879
Druh ASEP
C - Konferenční příspěvek (mezinárodní konf.)
Zařazení RIV
D - Článek ve sborníku
Název
Electrochemical determination of 2-nitrofluorene and investigation of its interaction with DNA
Tvůrce(i)
Skalová, Štěpánka (UFCH-W) Stávková, K. (CZ) Barek, J. (CZ) Vyskočil, V. (CZ)
Zdroj.dok.
Proceedings of the 12th International students conference Modern Analytical Chemistry. - Praha : VŠCHT, 2016 / Nesměrák K.
- ISBN 978-80-7444-044-1
Rozsah stran
s. 149-154
Poč.str.
6 s.
Forma vydání
Tištěná - P
Akce
ISC Modern Analytical Chemistry /12./
Datum konání
22.09.2016 - 23.09.2016
Místo konání
Prague
Země
CZ - Česká republika
Typ akce
WRD
Jazyk dok.
eng - angličtina
Země vyd.
CZ - Česká republika
Klíč. slova
DNA biosensors ; DNA interaction ; glassy carbon electrode
Vědní obor RIV
CG - Elektrochemie
Institucionální podpora
UFCH-W - RVO:61388955
UT WOS
000391253800028
Anotace
2-Nitrofluorene (2-NF) belongs to the group of nitrated polycyclic aromatic hydrocarbons. These compounds are categorized as environmental pollutants and they can manifest mutagenic and carcinogenic effects. A differential pulse voltammetric method at a glassy carbon electrode (GCE) was developed for sensitive determination of 2-NF. Under the optimum conditions found, a linear calibration dependence was obtained in the concentration range of 2 x 10(-7) - 1 x 10(-5) mol L-1, with the limit of quantification of 2 x 10 mol L-1. The practical applicability of this method was verified on the direct determination of 2-NF in a model sample of sand. Moreover, the mutual interaction between 2-NF and DNA was investigated using an electrochemical DNA biosensor (DNA-modified GCE). Cyclic voltammetry, electrochemical impedance spectroscopy, and square-wave voltammetry were employed in this study, confirming the formation of a 2-NF-DNA complex.
